Recombinant Human TPO
Induced proliferation of MO7e cells assay for Human TPO. Cell proliferation was measured to calculate the ED50, which is as expected less than 15 ng/ml.
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PODS® Growth Factors
The product is currently not available as PODS®, please check back in the future.Description
Thrombopoietin (TPO) is a growth factor that is produced by liver and kidney tissues. TPO binds the TPO receptor (CD110) to promote megakaryocyte maturation, differentiation, and the production of platelets.Activity Assay
Human TPO is fully biologically active when compared to standard. The activity is determined by the ability to induce MO7e cells proliferation and it is typically less than 15 ng/ml. This corresponds to an expected specific activity of 6.7 x 104 units/mg.AA Sequence
SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNELPNRTSG LLETNFTASA RTTGSGLLKW QQGFRAKIPG LLNQTSRSLD QIPGYLNRIH ELAlternative Names
TPO, Megakaryocyte Colony Stimulating Factor, c-MPL ligand, MGDF| Product Details | |
|---|---|
| Length | 338 aa |
| Molecular Weight | 36.3 kDa |
| Structure | Monomer |
| Source | Genetically modified CHO cells |
| Accession Number | P40225 |
| Purity | ≥90% determined by reducing and non-reducing SDS-PAGE |
| Endotoxin Level | ≤1.00 EU/μg as measured by kinetic LAL |
| Formulation | Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 5 mM sodium phosphate, 140 mg L-Arginine, pH 7.5 |
| Reconstitution | Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
| Stability and Storage |
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed. |
