Recombinant Human TPO

Code Description Price Qty
GFH216-5 Human TPO, 5 μg $115.00
GFH216-25 Human TPO, 25 μg $410.00
GFH216-100 Human TPO, 100 μg $1,090.00
GFH216-1000 Human TPO, 1 mg $6,550.00
Induced proliferation of MO7e cells assay for Human TPO. Cell proliferation was measured to calculate the ED50, which is as expected less than 15 ng/ml.

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Description

Thrombopoietin (TPO) is a growth factor that is produced by liver and kidney tissues. TPO binds the TPO receptor (CD110) to promote megakaryocyte maturation, differentiation, and the production of platelets.

Activity Assay

Human TPO is fully biologically active when compared to standard. The activity is determined by the ability to induce MO7e cells proliferation and it is typically less than 15 ng/ml. This corresponds to an expected specific activity of 6.7 x 104 units/mg.

AA Sequence

SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNELPNRTSG LLETNFTASA RTTGSGLLKW QQGFRAKIPG LLNQTSRSLD QIPGYLNRIH EL

Alternative Names

TPO, Megakaryocyte Colony Stimulating Factor, c-MPL ligand, MGDF
Product Details
Length 338 aa
Molecular Weight 36.3 kDa
Structure Monomer
Source Genetically modified CHO cells
Accession Number P40225
Purity ≥90% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 5 mM sodium phosphate, 140 mg L-Arginine, pH 7.5
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.