Recombinant Human Visfatin

Code Description Price Qty
GFH50-5 Human Visfatin, 5 μg $65.00
GFH50-25 Human Visfatin, 25 μg $165.00
GFH50-100 Human Visfatin, 100 μg $410.00
GFH50-1000 Human Visfatin, 1 mg $2,950.00
Induced expression of IL-6 from human PBMC cells for Human Visfatin. A significant increase in IL-6 is visible.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human Visfatin has a predicted Mw of 52.7 kDa.

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Description

Visfatin is an adipokine produced by visceral adipose tissue. Visfatin acts as a pro-inflammatory factor for peripheral blood mononuclear cells (PBMCs) and mimics the effects of insulin on adipocytes, monocytes and hepatocytes.

Activity Assay

Human Visfatin is fully biologically active when compared to standard. The activity is determined by the induced expression of IL-6 from Human PBMC cells.

AA Sequence

MPPNTSKVYS YFECREKKTE NSKLRKVKYE ETVFYGLQYI LNKYLKGKVV TKEKIQEAKD VYKEHFQDDV FNEKGWNYIL EKYDGHLPIE IKAVPEGFVI PRGNVLFTVE NTDPECYWLT NWIETILVQS WYPITVATNS REQKKILAKY LLETSGNLDG LEYKLHDFGY RGVSSQETAG IGASAHLVNF KGTDTVAGLA LIKKYYGTKD PVPGYSVPAA EHSTITAWGK DH

Alternative Names

PBEF
Product Details
Length 466 aa
Molecular Weight 52.7 kDa
Structure Monomer
Source E. coli
Accession Number P43490
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile 20 mM HCI at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.