Recombinant Human/Mouse TGF- β 3
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PODS® Growth Factors
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Description
Transforming growth factors (TGFs) are multifunctional peptides that regulate growth and differentiation in most cell types. The TGF- β family of proteins signal through serine/threonine kinase receptors. TGF-β isoforms (TGF-β1, -β2, and –β3) have overlapping, yet distinct biological actions in developing and adult tissues. TGF-β3 is an important factor in regulating cell adhesion and accelerating wound repair. TGF-β3 also functions during osteoblast proliferation, chemotaxis, and collagen synthesis.
Activity Assay
Human/Mouse TGF- β 3 is fully biologically active when compared to standard. The activity is determined by the dose dependent inhibition of IL-4-induced HT-2 cell proliferation, and it is typically less than 1 ng/ml.
AA Sequence
MALDTNYCFR NLEENCCVRP LYIDFRQDLG WKWVHEPKGY YANFCSGPCP YLRSADTTHS TVLGLYNTLN PEASASPCCV PQDLEPLTIL YYVGRTPKVE QLSNMVVKSC KCS
Alternative Names
None
| Product Details | |
|---|---|
| Length |
113/226 aa |
| Molecular Weight |
12.9/25.7 kDa |
| Structure |
Dimer |
| Source |
E. coli |
| Accession Number |
P10600/P17125 |
| Purity |
≥95% determined by reducing and non-reducing SDS-PAGE |
| Endotoxin Level |
≤0.1 EU/µg as measured by kinetic LAL |
| Formulation |
Lyophilized from a 0.2 µm filtered solution containing 0.1% Trifluoroacetic Acid (TFA) |
| Reconstitution |
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions. If a precipitate is observed, centrifuge the solution thoroughly and use only the soluble fraction (removing it from the precipitate). A 10% overfill has been added to compensate for any loss of protein in the precipitate. |
| Stability and Storage |
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed. |
