Recombinant Mouse FLT-3 Ligand (Animal-Free)
Induced proliferation of Aml5 cells assay for Mouse FLT-3 Ligand. Cell proliferation was measured to calculate the ED50, which is as expected less than 10 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Mouse FLT-3 Ligand has a predicted Mw of 18.6 kDa.
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Animal-FreeThis product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.
DescriptionFms-related tyrosine kinase 3 ligand (FLT-3 Ligand) is a growth factor that regulates hematopoietic cell proliferation. FLT-3 Ligand signalling is transmitted through the fms-related tyrosine kinase 3 (FLT-3) receptor. FLT-3 Ligand promotes the long-term expansion and differentiation of pro-B cells in the presence of Interleukin-7 (IL-7) or in combination of IL-7 and Interleukin-3 (IL-3).
Activity AssayMouse FLT-3 Ligand is fully biologically active when compared to standard. The activity is determined by the proliferation of Aml5 cells and it is typically less than 10 ng/ml. This corresponds to an expected specific activity of 1 x 105 units/mg.
AA SequenceMTPDCYFSHS PISSNFKVKF RELTDHLLKD YPVTVAVNLQ DEKHCKALWS LFLAQRWIEQ LKTVAGSKMQ TLLEDVNTEI HFVTSCTFQP LPECLRFVQT NISHLLKDTC TQLLALKPCI GKACQNFSRC LEVQCQPDSS TLLPPRSPIA LEATELPEPR PRQ
Alternative NamesFLt3 L, Fms-related tyrosine kinase 3 ligand, Flt3 ligand, SL cytokine, FL, flt3L, FLT3L
|Molecular Weight||18.6 kDa|
|Purity||≥95% determined by reducing and non-reducing SDS-PAGE|
|Endotoxin Level||≤1.00 EU/μg as measured by kinetic LAL|
|Formulation||Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)|
|Reconstitution||Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.|
|Stability and Storage||
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.