Recombinant Mouse FLT-3 Ligand

Code Description Price Qty
GFM6-2 Recombinant Mouse FLT-3 Ligand, 2 μg £50.00
GFM6-10 Recombinant Mouse FLT-3 Ligand, 10 μg £120.00
GFM6-100 Recombinant Mouse FLT-3 Ligand, 100 μg £357.50
GFM6-100x10 Recombinant Mouse FLT-3 Ligand, 100 μg x 10 £1,375.00
GFM6-1000 Recombinant Mouse FLT-3 Ligand, 1 mg £1,375.00
Induced proliferation of Aml5 cells assay for Mouse FLT-3 Ligand. Cell proliferation was measured to calculate the ED50, which is as expected less than 10 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Mouse FLT-3 Ligand has a predicted Mw of 18.6 kDa.

Achieve Purchasing Synergy

Request a quote at quotes@cellgs.com for 2 or more vials of any Growth Factor to receive a discount. Discounts range from 5 to 20% according to the size of your order (offer excludes the largest pack size - please contact us).

PODS Growth Factors

The product is current not available as PODS™, please check back in the future.

Description

Fms-related tyrosine kinase 3 ligand (FLT-3 Ligand) is a growth factor that regulates hematopoietic cell proliferation. FLT-3 Ligand signalling is transmitted through the fms-related tyrosine kinase 3 (FLT-3) receptor. FLT-3 Ligand promotes the long-term expansion and differentiation of pro-B cells in the presence of Interleukin-7 (IL-7) or in combination of IL-7 and Interleukin-3 (IL-3).

Activity Assay

Mouse FLT-3 Ligand is fully biologically active when compared to standard. The activity is determined by the proliferation of Aml5 cells and it is typically less than 10 ng/ml. This corresponds to an expected specific activity of 1 x 105 units/mg.

AA Sequence

MTPDCYFSHS PISSNFKVKF RELTDHLLKD YPVTVAVNLQ DEKHCKALWS LFLAQRWIEQ LKTVAGSKMQ TLLEDVNTEI HFVTSCTFQP LPECLRFVQT NISHLLKDTC TQLLALKPCI GKACQNFSRC LEVQCQPDSS TLLPPRSPIA LEATELPEPR PRQ

Alternative Names

FLt3 L, Fms-related tyrosine kinase 3 ligand, Flt3 ligand, SL cytokine, FL, flt3L, FLT3L
Product Details
Length 163 aa
Molecular Weight 18.6 kDa
Structure Monomer
Source E. coli
Accession Number P49772
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.