Recombinant Mouse GDNF (Animal-Free)
Induced proliferation of C6 cells assay for Mouse GDNF. Cell proliferation was measured to calculate the ED50.
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PODS® Growth Factors
The product is currently not available as PODS®, please check back in the future.Animal-Free
This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.Description
Glial cell-derived neurotrophic factor (GDNF) is a neurotrophic factor that signals through a multicomponent receptor system to activate receptor tyrosine kinase RET signaling. GDNF promotes dopamine uptake, prevents motor neuron apoptosis, and supports the survival and differentiation of neurons.Activity Assay
Mouse GDNF (Animal-Free) is fully biologically active when compared to standard. The activity is determined by a proliferation assay using C6 cells.AA Sequence
MSPDKQAAL PRRENRNRQAA AASPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCESAETMY DKILKNLSRS RRLTSDKVGQ ACCRPVAFDD DLSFLDDNLV YHILRKHSAK RCGCIAlternative Names
ATF, HFB1-GDNF, ATF1, ATF-1, astrocyte-derived trophic factor 2, glial cell line derived neurotrophic factor| Product Details | |
|---|---|
| Length | 135 / 270 aa |
| Molecular Weight | 15.1 / 30.2 kDa |
| Structure | Dimer |
| Source | E. coli |
| Accession Number | P48540 |
| Purity | ≥95% determined by reducing and non-reducing SDS-PAGE |
| Endotoxin Level | ≤1.00 EU/μg as measured by kinetic LAL |
| Formulation | Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA) |
| Reconstitution | Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
| Stability and Storage |
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed. |
