Recombinant Mouse GM-CSF

Code Description Price Qty
GFM15-5 Mouse GM-CSF, 5 μg $65.00
GFM15-20 Mouse GM-CSF, 20 μg $165.00
GFM15-100 Mouse GM-CSF, 100 μg $410.00
GFM15-1000 Mouse GM-CSF, 1 mg $3,060.00
Induced proliferation of FDCP-1 cells assay for Mouse GM-CSF. Cell proliferation was measured to calculate the ED50, which is as expected less than 50 pg/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Mouse GM-CSF has a predicted Mw of 14.3 kDa.

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Description

Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) is hematopoietic growth factor produced by endothelial cells, monocytes, fibroblasts, and T cells. GM-CSF stimulates the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells. GM-CSF promotes immune system development and regulates neutrophil function during infection. Human and mouse GM-CSF show no cross-reactivity.

Activity Assay

Mouse GM-CSF is fully biologically active when compared to standard. The activity is determined by a proliferation assay using FDC-P1 cells and it is typically less than 50 pg/ml. This corresponds to an expected specific activity of 2 x 107 units/mg.

AA Sequence

MAPTRSPITV TRPWKHVEAI KEALNLLDDM PVTLNEEVEV VSNEFSFKKL TCVQTRLKIF EQGLRGNFTK LKGALNMTAS YYQTYCPPTP ETDCETQVTT YADFIDSLKT FLTDIPFECK KPVQK

Alternative Names

Granulocyte-Macrophage Colony-Stimulating Factor, GMCSF, CSF-2, MGI1GM, colony stimulating factor 2 (granulocyte-macrophage), colony-stimulating factor, CSF-2, MGIIGM, sargramostim, molgramostin
Product Details
Length 125 aa
Molecular Weight 14.3 kDa
Structure Monomer
Source E. coli
Accession Number P01587
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM acetic acid
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.