Recombinant Mouse IGF-2 (Animal-Free)
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Animal-FreeThis product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.
DescriptionInsulin-like Growth Factor 2 (IGF-2) is an important fetal growth hormone made by theca cells during gestation. IGF-2 engages the IGF-1 receptor (IGF1R) to mediate embryonic growth. IGF-2 also binds the sink IGF-2 receptor (IGF2R) leading to IGF-2 degradation.
Activity AssayMouse IGF-2 (Animal-Free) is fully biologically active when compared to standard. The activity is determined by the ability to induce FDC-P1 cells proliferation and it is typically less than 50 ng/ml. This corresponds to an expected specific activity higher than 2.0 x 104 units/mg.
AA SequenceAYGPGETLCG GELVDTLQFV CSDRGFYFSR PSSRANRRSR GIVEECCFRS CDLALLETYC ATPAKSE
Alternative NamesInsulin-like Growth Factor 2, somatamedin A, IGF-II
|Molecular Weight||7.4 kDa|
|Purity||≥95% determined by reducing and non-reducing SDS-PAGE|
|Endotoxin Level||≤1.00 EU/μg as measured by kinetic LAL|
|Formulation||Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)|
|Reconstitution||Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. Do not vortex. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.|
|Stability and Storage||
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.