Recombinant Mouse IHH (Animal-Free)
Induced alkaline phosphatase activity of CCL-226 cells assay for Mouse IHH. Cell proliferation was measured to calculate the ED50.
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Animal-FreeThis product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.
DescriptionIndian hedgehog (IHH) is an essential signaling factor that is secreted in the gut, cartilage, and bone during embryonic development. IHH acts through the patched (PTC) receptor to induce transcriptional changes important for bone and cartilage development. IHH also induces the expression of parathyroid hormone-related peptide (PTHrP), which in turn mediates IHH activity during chondrocyte differentiation, forming a negative feedback loop.
Activity AssayMouse IHH (Animal-Free) is fully biologically active when compared to standard. The activity is determined by the induced alkaline phosphatase activity of CCL-226 cells.
AA SequenceMIIGPGRVVG SRRRPPRKLV PLAYKQFSPN VPEKTLGASG RYEGKIARSS ERFKELTPNY NPDIIFKDEE NTGADRLMTQ RCKDRLNSLA ISVMNQWPGV KLRVTEGWDE DGHHSEESLH YEGRAVDITT SDRDRNKYGL LARLAVEAGF DWVYYESKAH VHCSVKSEHS AAAKTGG
Alternative NamesIndian Hedgehog, HHG-2
|Molecular Weight||19.9 kDa|
|Purity||≥95% determined by reducing and non-reducing SDS-PAGE|
|Endotoxin Level||≤1.00 EU/μg as measured by kinetic LAL|
|Formulation||Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)|
|Reconstitution||Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile 10 mM HCI at 0.1 mg/ml, which can be further diluted into other aqueous solutions.|
|Stability and Storage||
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.