Recombinant Mouse IL-10

Code Description Price Qty
GFM84-2 Mouse IL-10, 2 μg $65.00
GFM84-10 Mouse IL-10, 10 μg $165.00
GFM84-100 Mouse IL-10, 100 μg $755.00
GFM84-1000 Mouse IL-10, 1 mg $5,240.00
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Mouse IL-10 has a predicted Mw of 18.9 kDa.

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Description

Interleukin-10 (IL-10) is an anti-inflammatory cytokine produced by macrophages and type 2 T helper (Th2) cells. IL-10 inhibits the production of pro-inflammatory cytokines such as interferon-γ (IFN-γ), Tumor Necrosis Factor α (TNF-α), interleukin-2 (IL-2), interleukin-3 (IL-3), interleukin-4 (IL-4), and Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF), made by macrophages and regulatory T cells. IL-10 also suppresses antigen presentation on antigen presenting cells, and enhances the survival, proliferation, and antibody production of B cells. Human IL-10 can act on mouse cells, while mouse IL-10 is not active on human cells.

Activity Assay

Mouse IL-10 is fully biologically active when compared to standard. The activity is determined by the ability to induce MC/9 cells proliferation and it is typically less than 5 ng/ml. This corresponds to an expected specific activity of 2.0 x 105 units/mg.

AA Sequence

MSRGQYSRED NNCTHFPVGQ SHMLLELRTA FSQVKTFFQT KDQLDNILLT DSLMQDFKGY LGCQALSEMI QFYLVEVMPQ AEKHGPEIKE HLNSLGEKLK TLRMRLRRCH RFLPCENKSK AVEQVKSDFN KLQDQGVYKA MNEFDIFINC IEAYMMIKMK S

Alternative Names

Interleukin-10, interleukin 10, IL10, IL 10, B-TCGF, CSIF, TGIF, cytokine synthesis inhibitory factor
Product Details
Length 161 aa
Molecular Weight 18.9 kDa
Structure Non-Covalent Homodimer
Source E. coli
Accession Number P18893
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.