Recombinant Mouse IL-12

Code Description Price Qty
GFM25-2 Mouse IL-12, 2 μg $65.00
GFM25-10 Mouse IL-12, 10 μg $165.00
GFM25-100 Mouse IL-12, 100 μg $1,310.00
GFM25-1000 Mouse IL-12, 1 mg $7,860.00
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Mouse IL-12 has comprised with one subunit which is predicted Mw of 35.8 kDa and one subunit which has predict

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Description

Interleukin-12 (IL-12) is an inducer of cell-mediated immunity and is produced by dendritic cells, macrophages, and B cells after antigen stimulation. IL-12 functions to promote naive T cell differentiation into the type 1 T helper cell (Th1) lineage. IL-12 also stimulates the production of interferon-γ (IFN-γ) and Tumor Necrosis Factor α (TNF-α) from T cells and Natural Killer cells. Mouse IL-12 is active on human and mouse cells.

Activity Assay

Mouse IL-12 is fully biologically active when compared to standard. The activity is determined by the ability to induce expression of CD25 by mouse primary T cells and it is typically less than 20 ng/ml. This corresponds to an expected specific activity of 5 x 104 units/mg.

AA Sequence

p35: MRVIPVSGPA RCLSQSRNLL KTTDDMVKTA REKLKHYSCT AEDIDHEDIT RDQTSTLKTC LPLELHKNES CLATRETSST TRGSCLPPQK TSLMMTLCLG SIYEDLKMYQ TEFQAINAAL QNHNHQQIIL DKGMLVAIDE LMQSLNHNGE TLRQKPPVGE ADPYRVKMKL CILLHAFSTR VVTINRVMGY LSSA p40: MWELEKDVYV VEVDWTPDAP GETVNL

Alternative Names

Interleukin-12, interleukin 12, IL12, IL 12, NKSF, CLMF
Product Details
Length p35: 194 aa, p40: 313 aa total: 507 aa
Molecular Weight p35: 21.8 KDa, p40: 35.8 kDa, total: 57.6 kDa
Structure Heterodimer
Source HEK 293 cells
Accession Number P43432/P43431
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.