Recombinant Mouse IL-17A

Code Description Price Qty
GFM59-5 Mouse IL-17A, 5 μg $65.00
GFM59-25 Mouse IL-17A, 25 μg $165.00
GFM59-100 Mouse IL-17A, 100 μg $410.00
GFM59-1000 Mouse IL-17A, 1 mg $2,950.00
Induced production of mouse IL-6 by NIH-3T3 cells. Production of mouse IL-6 was measured to calculate the ED50, which is as expected less than 5 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Mouse IL-17A has a predicted Mw of 30.2 kDa (each monomer is 15.1 kDa).

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Description

Interleukin-17A (IL-17A), also known as CTLA-8, is a member of the IL-17 family of proteins. IL-17A is a proinflammatory cytokine that is secreted by activated CD4+ and CD8+ T lymphocytes. IL-17A acts through its receptor, IL-17R, to promote increased cytokine and chemokine secretion. In turn, the cytokines and chemokines mediate the immunoregulatory function of IL-17A by promoting the proliferation, maturation, and chemoattraction of neutrophils to inflammatory sites. Elevated levels of IL-17A are associated with rheumatoid arthritis, airway inflammation, allograft rejection, inflammatory bowel disease, psoriasis, cancer, and multiple sclerosis. Human, mouse, and rat IL-17A show activity on mouse cells.

Activity Assay

Mouse IL-17A is fully biologically active when compared to standard. The activity is determined by the ability to produce mouse IL-6 by NIH-3T3 cells and it is typically less than 5 ng/ml. This corresponds to an expected specific activity of 2 x 105 units/mg.

AA Sequence

MAAIIPQSSA CPNTEAKDFL QNVKVNLKVF NSLGAKVSSR RPSDYLNRST SPWTLHRNED PDRYPSVIWE AQCRHQRCVN AEGKLDHHMN SVLIQQEILV LKREPESCPF TFRVEKMLVG VGCTCVASIV RQAA

Alternative Names

Interleukin-17A, interleukin 17A, IL17A, IL 17A
Product Details
Length 134 / 268 aa
Molecular Weight 15.1 / 30.2 kDa
Structure Monomer
Source E. coli
Accession Number Q62386
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.