Recombinant Mouse IL-22 (Animal-Free)
Induced production of IL-10 by human COLO-205 cells. Production of IL-10 was measured to calculate the ED50, which is as expected less than 1 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Mouse IL-22 has a predicted Mw of 16.8 kDa.
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Animal-FreeThis product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.
DescriptionInterleukin-22 (IL-22) is an IL-10 family member that is produced by activated dendritic cells and T lymphocytes. IL-22 signals via a heteroduplex receptor consisting of IL-22R and IL-10Rβ chains. IL-22 is a potent mediator of cellular inflammatory responses.
Activity AssayMouse IL-22 (Animal-Free) is fully biologically active when compared to standard. The activity is determined by the ability to induce production of IL-10 from human COLO-205 cells and it is typically less than 1 ng/ml. This corresponds to an expected specific activity of 1 x 106 units/mg.
AA SequenceMLPVNTRCKL EVSNFQQPYI VNRTFMLAKE ASLADNNTDV RLIGEKLFRG VSAKDQCYLM KQVLNFTLED VLLPQSDRFQ PYMQEVVPFL TKLSNQLSSC HISGDDQNIQ KNVRRLKETV KKLGESGEIK AIGELDLLFM SLRNACV
Alternative NamesInterleukin-22, interleukin 22, IL22, IL 22, IL-TIF
|Molecular Weight||16.8 kDa|
|Purity||≥95% determined by reducing and non-reducing SDS-PAGE|
|Endotoxin Level||≤1.00 EU/μg as measured by kinetic LAL|
|Formulation||Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)|
|Reconstitution||Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.|
|Stability and Storage||
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.