Recombinant Mouse IL-4 (Animal-Free)

Code Description Price Qty
GFM18AF-5 Mouse IL-4 (Animal-Free), 5 μg $70.00
GFM18AF-20 Mouse IL-4 (Animal-Free), 20 μg $180.00
GFM18AF-100 Mouse IL-4 (Animal-Free), 100 μg $455.00
GFM18AF-1000 Mouse IL-4 (Animal-Free), 1 mg $3,715.00
Induced proliferation of HT-2 cells assay for Mouse IL-4. Cell proliferation was measured to calculate the ED50, which is as expected less than 20 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human Mouse IL-4 has a predicted Mw of 13.7 kDa.

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PODS® Growth Factors

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Animal-Free

This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.

Description

Interleukin-4 (IL-4) is an immunomodulatory cytokine that functions to induce naive helper T cells to differentiate into type 2 T helper (Th2) cells. Th2 cells subsequently produce more IL-4 in a positive feedback loop. IL-4 also promotes immunoglobulin IgG to IgE isotype switching on B cells. IL-4 binds the IL-4Rα receptor to activate STAT6 signaling.

Activity Assay

Mouse IL-4 (Animal-Free) is fully biologically active when compared to standard. The activity is determined by the ability to induce HT-2 cells proliferation and it is typically less than 20 ng/ml. This corresponds to an expected specific activity of 5.0 x 104 units/mg.

AA Sequence

MAPMTQTTPL KTSWVNCSNM IDEIITHLKQ PPLPLLDFNN LNGEDQDILM ENNLRRPNLE AFNRAVKSLQ NASAIESILK NLLPCLPLAT AAPTRHPIHI KDGDWNEFRR KLTFYLKTLE NAQAQQTTLS LAIF

Alternative Names

Interleukin-3, interleukin 3, IL3, IL 3, MCGF, Multi-CSF, HCGF, P-cell stimulation factor
Product Details
Length 134 aa
Molecular Weight 15.2 kDa
Structure Monomer
Source E. coli
Accession Number P08700
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile 10 mM acetic acid at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.