Recombinant Mouse IL-5 (Animal-Free)
Induced proliferation of TF-1 cells for Mouse IL-5. Cell proliferation was measured to calculate the ED50, which is as expected less than 2 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Mouse IL-5 has a predicted Mw of 26.3 kDa (each monomer is 13.1 kDa).
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Animal-FreeThis product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.
DescriptionInterleukin-5 (IL-5) is a hematopoietic growth factor that is expressed in type 2 T helper (Th2) cells, mast cells, and eosinophils. IL-5 acts through the IL-5 receptor (IL-5R), stimulates B cell growth, and mediates eosinophil activation. Human and mouse IL-5 show cross-reactivity.
Activity AssayMouse IL-5 (Animal-Free) is fully biologically active when compared to standard. The activity is determined by the ability to induce TF-1 cells proliferation and it is typically less than 2 ng/ml. This corresponds to an expected specific activity of 5 x 105 units/mg.
AA SequenceMEIPMSTVVK ETLTQLSAHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEMLFQNL SLIKKYIDRQ KEKCGEERRR TRQFLDYLQE FLGVMSTEWA MEG
Alternative NamesInterleukin-5, interleukin 5, IL5, IL 5, EDF, BCDFII, TRF
|Length||113 / 226 aa|
|Molecular Weight||13.1 / 26.3 kDa|
|Purity||≥95% determined by reducing and non-reducing SDS-PAGE|
|Endotoxin Level||≤1.00 EU/μg as measured by kinetic LAL|
|Formulation||Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 20 mM sodium bicarbonate, pH 9.0|
|Reconstitution||Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.|
|Stability and Storage||
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.