Recombinant Mouse IL-5
Induced proliferation of TF-1 cells for Mouse IL-5. Cell proliferation was measured to calculate the ED50, which is as expected less than 2 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Mouse IL-5 has a predicted Mw of 26.3 kDa (each monomer is 13.1 kDa).
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PODS® Growth Factors
The product is currently not available as PODS®, please check back in the future.Description
Interleukin-5 (IL-5) is a hematopoietic growth factor that is expressed in type 2 T helper (Th2) cells, mast cells, and eosinophils. IL-5 acts through the IL-5 receptor (IL-5R), stimulates B cell growth, and mediates eosinophil activation. Human and mouse IL-5 show cross-reactivity.Activity Assay
Mouse IL-5 is fully biologically active when compared to standard. The activity is determined by the ability to induce TF-1 cells proliferation and it is typically less than 2 ng/ml. This corresponds to an expected specific activity of 5 x 105 units/mg.AA Sequence
MEIPMSTVVK ETLTQLSAHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEMLFQNL SLIKKYIDRQ KEKCGEERRR TRQFLDYLQE FLGVMSTEWA MEGAlternative Names
Interleukin-5, interleukin 5, IL5, IL 5, EDF, BCDFII, TRF| Product Details | |
|---|---|
| Length | 113 / 226 aa |
| Molecular Weight | 13.1 / 26.3 kDa |
| Structure | Dimer |
| Source | E. coli |
| Accession Number | P04401 |
| Purity | ≥95% determined by reducing and non-reducing SDS-PAGE |
| Endotoxin Level | ≤1.00 EU/μg as measured by kinetic LAL |
| Formulation | Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 20 mM sodium bicarbonate, pH 9.0 |
| Reconstitution | Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
| Stability and Storage |
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed. |
