Recombinant Mouse IL-5

Code Description Price Qty
GFM20-5 Mouse IL-5, 5 μg $65.00
GFM20-25 Mouse IL-5, 25 μg $165.00
GFM20-100 Mouse IL-5, 100 μg $410.00
GFM20-1000 Mouse IL-5, 1 mg $2,950.00
Induced proliferation of TF-1 cells for Mouse IL-5. Cell proliferation was measured to calculate the ED50, which is as expected less than 2 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Mouse IL-5 has a predicted Mw of 26.3 kDa (each monomer is 13.1 kDa).

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Description

Interleukin-5 (IL-5) is a hematopoietic growth factor that is expressed in type 2 T helper (Th2) cells, mast cells, and eosinophils. IL-5 acts through the IL-5 receptor (IL-5R), stimulates B cell growth, and mediates eosinophil activation. Human and mouse IL-5 show cross-reactivity.

Activity Assay

Mouse IL-5 is fully biologically active when compared to standard. The activity is determined by the ability to induce TF-1 cells proliferation and it is typically less than 2 ng/ml. This corresponds to an expected specific activity of 5 x 105 units/mg.

AA Sequence

MEIPMSTVVK ETLTQLSAHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEMLFQNL SLIKKYIDRQ KEKCGEERRR TRQFLDYLQE FLGVMSTEWA MEG

Alternative Names

Interleukin-5, interleukin 5, IL5, IL 5, EDF, BCDFII, TRF
Product Details
Length 113 / 226 aa
Molecular Weight 13.1 / 26.3 kDa
Structure Dimer
Source E. coli
Accession Number P04401
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 20 mM sodium bicarbonate, pH 9.0
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.