Recombinant Mouse IL-7

Code Description Price Qty
GFM3-2 Mouse IL-7, 2 μg $65.00
GFM3-10 Mouse IL-7, 10 μg $165.00
GFM3-100 Mouse IL-7, 100 μg $635.00
GFM3-1000 Mouse IL-7, 1 mg $5,240.00

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Description

Interleukin-7 (IL-7) is a hematopoietic cytokine that is an important regulator of B and T cell development. IL-7 is secreted by bone marrow and thymic stromal cells, dendritic cells, intestinal epithelial cells, hepatocytes, and keratinocytes. IL-7 signals through the interleukin-7 receptor (IL-7R) to promote the differentiation of hematopoietic stem cells into T cells, B cells, and Natural Killer cells. IL-7 is also a regulator of intestinal mucosal lymphocyte proliferation. Human and mouse IL-7 show species cross-reactivity.

Activity Assay

Mouse IL-7 is fully biologically active when compared to standard. The activity is determined by the ability to induce mouse 2E8 cells proliferation and it is typically less than 1 ng/ml. This corresponds to an expected specific activity of 1 x 106 units/mg.

AA Sequence

MECHIKDKEG KAYESVLMIS IDELDKMTGT DSNCPNNEPN FFRKHVCDDT KEAAFLNRAA RKLKQFLKMN ISEEFNVHLL TVSQGTQTLV NCTSKEEKNV KEQKKNDACF LKRLLREIKT CWNKILKGSI

Alternative Names

Interleukin-7, interleukin 7, IL7, IL 7, lymphopoietin 1, LP-1, pre-B cell factor
Product Details
Length 130 aa
Molecular Weight 15 kDa
Structure Monomer
Source E. coli
Accession Number P10168
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile 10 mM acetic acid at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.