Recombinant Mouse LIF (Animal-Free)

Induced production of IL-6 from M1 cells by Mouse LIF. Cell proliferation was measured to calculate the ED50, which is as expected less than 1 ng/ml.

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Animal-Free

This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.

Description

Leukemia Inhibitory Factor (LIF) is a member of the interleukin-6 (IL-6) family that is made by a variety of adult and embryonic tissues. LIF signals through the glycoprotein 130 (gp130)/LIF receptor (LIFR) heterodimer to activate STAT3 and MAPK signaling. LIF functions during hematopoietic differentiation, neuronal cell differentiation, kidney development, and inflammatory processes. The application of mouse LIF to long-term culture systems promotes mouse embryonic stem cell (ESC) self-renewal and pluripotency, similar to the functional activity of FGF-2 in human ESC cell culture systems.

Activity Assay

Mouse LIF (Animal-Free) is fully biologically active when compared to standard. The activity is determined by the induce production of IL-6 from M1 cells and it is typically less than 1 ng/ml. This corresponds to an expected specific activity of 1 x 106 units/mg.

AA Sequence

MSPLPITPVN ATCAIRHPCH GNLMNQIKNQ LAQLNGSANA LFISYYTAQG EPFPNNVEKL CAPNMTDFPS FHGNGTEKTK LVELYRMVAY LSASLTNITR DQKVLNPTAV SLQVKLNATI DVMRGLLSNV LCRLCNKYRV GHVDVPPVPD HSDKEAFQRK KLGCQLLGTY KQVISVVVQA F

Alternative Names

Leukocyte Inhibitory Factor, leukemia inhibitory factor, cholinergic differentiation factor
Product Details
Length 181 aa
Molecular Weight 19.8 kDa
Structure Monomer
Source E. coli
Accession Number P09056
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile 10 mM acetic acid at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.