Recombinant Mouse MCSF

Code Description Price Qty
GFM8-2 Mouse MCSF, 2 μg $65.00
GFM8-10 Mouse MCSF, 10 μg $165.00
GFM8-100 Mouse MCSF, 100 μg $530.00
GFM8-1000 Mouse MCSF, 1 mg $4,260.00
Induced proliferation of NFS-60 cells assay for Mouse MCSF. Cell proliferation was measured to calculate the ED50, which is as expected less than 10 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Mouse MCSF has a predicted Mw of 36.4 kDa (each monomer is 18.2 kDa).

Achieve Purchasing Synergy

Request a quote at quotes@cellgs.com for 2 or more vials of any Growth Factor to receive a discount. Discounts range from 5 to 20% according to the size of your order.

PODS® Growth Factors

The product is currently not available as PODS®, please check back in the future.

Description

Macrophage Colony Stimulating Factor (MCSF) is a hematopoietic growth factor that is widely produced by a variety of cells. MCSF stimulates the proliferation and differentiation of hematopoietic stem cells into monocyte and macrophage cell types. MCSF also acts through the colony stimulating factor 1 receptor (CSF1R) to modulate processes involved in immunology, bone metabolism, fertility, and pregnancy. Human MCSF shows activity on mouse cells, however mouse MCSF shows no activity on human cells.

Activity Assay

Mouse MCSF is fully biologically active when compared to standard. The activity is determined by the ability to induce NFS-60 cells proliferation and it is typically less than 10 ng/ml. This corresponds to an expected specific activity of 1 x 105 units/mg.

AA Sequence

MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP

Alternative Names

Macrophage Colony Stimulating Factor, M-CSF, MGI-IM, CSF-1
Product Details
Length 156 / 312 aa
Molecular Weight 18.2 / 36.4 kDa
Structure Monomer
Source E. coli
Accession Number P07141
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.

Citations

Thomas L, Rao Z, Gerstmeier J, Raasch M, Weinigel C, Rummler S, Menche D, Muller R, Pergola C, Mosig A, et al. Selective upregulation of TNFα expression in classically-activated human monocyte-derived macrophages (M1) through pharmacological interferen