Recombinant Mouse MIP-1 α / CCL3

Code Description Price Qty
GFM42-2 Mouse MIP-1 α, 2 μg $65.00
GFM42-10 Mouse MIP-1 α, 10 μg $165.00
GFM42-100 Mouse MIP-1 α, 100 μg $410.00
GFM42-1000 Mouse MIP-1 α, 1 mg $2,950.00
Induced chemotaxis of THP-1 cells assay for Mouse MIP-1 α. Cells that migrated were counted using a luminescent substrate. Migration over basal levels was reported in response to Mouse MIP-1 α starting at 10 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Mouse MIP-1 α has a predicted Mw of 7.9 kDa.

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Description

Macrophage Inflammatory Protein 1 α (MIP-1 α), also known as CCL3, is a cytokine produced by macrophages. MIP-1 α binds the chemokine receptors CCR1, CCR4 and CCR5 to induce inflammatory responses, including the recruitment of granulocytes and neutrophil superoxide production. The MIP-1 α and MIP-1 β heterodimer exhibits antiviral activity against the human immunodeficiency virus 1 (HIV-1).

Activity Assay

Mouse MIP-1 α is fully biologically active when compared to standard. The activity is determined by the ability to induce chemotaxis of THP-1 cells and it is typically less than 100 ng/ml.

AA Sequence

ASLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPGV IFLTKRSRQV CADPSEEWVQ KYVSDLELSA

Alternative Names

Monocyte Chemotactic Protein 1, MIP-1 alpha, CCL3
Product Details
Length 70 aa
Molecular Weight 7.8 kDa
Structure Monomer
Source E. coli
Accession Number P10147
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.