Recombinant Mouse MIP-1 α / CCL3
Induced chemotaxis of THP-1 cells assay for Mouse MIP-1 α. Cells that migrated were counted using a luminescent substrate. Migration over basal levels was reported in response to Mouse MIP-1 α starting at 10 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Mouse MIP-1 α has a predicted Mw of 7.9 kDa.
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DescriptionMacrophage Inflammatory Protein 1 α (MIP-1 α), also known as CCL3, is a cytokine produced by macrophages. MIP-1 α binds the chemokine receptors CCR1, CCR4 and CCR5 to induce inflammatory responses, including the recruitment of granulocytes and neutrophil superoxide production. The MIP-1 α and MIP-1 β heterodimer exhibits antiviral activity against the human immunodeficiency virus 1 (HIV-1).
Activity AssayMouse MIP-1 α is fully biologically active when compared to standard. The activity is determined by the ability to induce chemotaxis of THP-1 cells and it is typically less than 100 ng/ml.
AA SequenceASLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPGV IFLTKRSRQV CADPSEEWVQ KYVSDLELSA
Alternative NamesMonocyte Chemotactic Protein 1, MIP-1 alpha, CCL3
|Molecular Weight||7.8 kDa|
|Purity||≥95% determined by reducing and non-reducing SDS-PAGE|
|Endotoxin Level||≤1.00 EU/μg as measured by kinetic LAL|
|Formulation||Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)|
|Reconstitution||Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.|
|Stability and Storage||
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.