Recombinant Mouse MIP-3 α / CCL20

Code Description Price Qty
GFM51-5 Mouse MIP-3 α, 5 μg $65.00
GFM51-20 Mouse MIP-3 α, 20 μg $165.00
GFM51-100 Mouse MIP-3 α, 100 μg $410.00
GFM51-1000 Mouse MIP-3 α, 1 mg $2,950.00
Induced chemotaxis of THP-1 cells assay for Mouse MIP-3 α. Cells that migrated were counted using a luminescent substrate. Migration over basal levels was reported in response to Mouse MIP-3 α starting at 5 ng/ml.

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Description

Macrophage Inflammatory Protein 3 α (MIP-3 α), also known as CCL20, is expressed in the liver, lungs, lymph nodes, and peripheral blood lymphocytes. MIP-3 α expression is strongly induced by inflammatory signals, and downregulated by the anti-inflammatory cytokine interleukin-10 (IL-10). MIP-3 α signals through the G protein-coupled receptor CCR6 to function as a chemoattractant to lymphocytes and dendritic cells.

Activity Assay

Mouse MIP-3 α is fully biologically active when compared to standard. The activity is determined by the ability to induce chemotaxis of THP-1 cells.

AA Sequence

ASNFDCCLGY TDRILHPKFI VGFTRQLANE GCDINAIIFH TKKKLSVCAN PKQTWVKYIV RLLSKKVKNM

Alternative Names

Monocyte Chemotactic Protein 3, MIP-3 alpha, CCL20
Product Details
Length 70 aa
Molecular Weight 8.0 kDa
Structure Monomer
Source E. coli
Accession Number P78556
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.