Recombinant Mouse NGF-β (Animal-Free)
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PODS® Growth Factors
The product is currently not available as PODS®, please check back in the future.Animal-Free
This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.Description
Nerve Growth Factor β (NGF-β) is a neurotrophic factor that is important for the development and maintenance of sensory and sympathetic neurons. NGF-β signals through the low affinity nerve growth factor receptor (LNGFR) and the tropomyosin receptor kinase A (TrkA) to activate PI3K, Ras, and PLC signaling pathways. NGF-β is also involved in the growth, differentiation, and survival of B lymphocytes. Human, mouse, and rat NGF-β proteins are cross-reactive.Activity Assay
Mouse NGF-β (Animal-Free) is fully biologically active when compared to standard. The activity is determined by the ability to induce TF-1 cells proliferation and it is typically less than 5 ng/ml. This corresponds to an expected specific activity of 2.0 x 105 units/mg.AA Sequence
MSSTHPVFHM GEFSVCDSVS VWVGDKTTAT DIKGKEVTVL AEVNINNSVF RQYFFETKCR ASNPVESGCR GIDSKHWNSY CTTTHTFVKA LTTDEKQAAW RFIRIDTACV CVLSRKATRR GAlternative Names
Nerve Growth Factor β, β-nerve growth factor, bNGF, NGFB| Product Details | |
|---|---|
| Length | 121 / 242 aa |
| Molecular Weight | 13.6 / 27.2 kDa |
| Structure | Dimer |
| Source | E. coli |
| Accession Number | Q6LDU8 |
| Purity | ≥95% determined by reducing and non-reducing SDS-PAGE |
| Endotoxin Level | ≤1.00 EU/μg as measured by kinetic LAL |
| Formulation | Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA) |
| Reconstitution | Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
| Stability and Storage |
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed. |
