Recombinant Mouse SDF-1 β / CXCL12b

Code Description Price Qty
GFM67-2 Mouse SDF-1 β, 2 μg $65.00
GFM67-10 Mouse SDF-1 β, 10 μg $165.00
GFM67-100 Mouse SDF-1 β, 100 μg $410.00
GFM67-1000 Mouse SDF-1 β, 1 mg $2,950.00
Induced chemotaxis of neutrophils assay for Mouse SDF-1 β. Cells that migrated were counted using a luminescent substrate. Migration over basal levels was reported in response to Mouse SDF-1 β starting at 1000 ng/ml.

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Description

Stromal cell-derived factor-1 β (SDF-1 β), also known as CXCL12b, is one of two SDF-1 splice variants made by a wide variety of cells upon stimulation by inflammatory cytokines such as TNF, IL-1, and LPS. SDF-1 β signals through the G protein-coupled receptor CXCR4 to recruit activated leukocytes.

Activity Assay

Mouse SDF-1 β is fully biologically active when compared to standard. The activity is determined by the ability to induce chemotaxis of human neutrophils.

AA Sequence

KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK

Alternative Names

Monocyte Chemotactic Protein 1, CCL2, JE, MCAF
Product Details
Length 68 aa
Molecular Weight 8.0 kDa
Structure Monomer
Source E. coli
Accession Number P48061 (2)
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.