Recombinant Mouse SHH

Code Description Price Qty
GFM55-5 Mouse SHH, 5 μg $65.00
GFM55-25 Mouse SHH, 25 μg $165.00
GFM55-100 Mouse SHH, 100 μg $410.00
GFM55-1000 Mouse SHH, 1 mg $2,730.00
Induced alkaline phosphatase activity of CCL-226 cells assay in the presence of 1 μM retinoic acid. Alkaline phosphatase was measured to calculate the ED50.

Achieve Purchasing Synergy

Request a quote at quotes@cellgs.com for 2 or more vials of any Growth Factor to receive a discount. Discounts range from 5 to 20% according to the size of your order.

PODS® Growth Factors

The product is currently not available as PODS®, please check back in the future.

Description

Sonic hedgehog (SHH) is a member of a small group of hedgehog secreted proteins that are essential for development in both vertebrates and invertebrates. There are three mammalian hedgehog homologues, sonic, desert, and indian, that signal via the Patched-1 and Patched-2 receptors. SHH is a morphogen that is essential during vertebrate organogenesis and adult stem cell division.

Activity Assay

Mouse SHH is fully biologically active when compared to standard. The activity is determined by the ability to induce alkaline phophatase activity of CCL-226 cells.

AA Sequence

MSPAPPACDL RVLSKLLRDS HVLHSRLSQC PEVHPLPTPV LLPAVDFSLG EWKTQMEETK AQDILGAVTL LLEGVMAARG QLGPTCLSSL LGQLSGQVRL LLGALQSLLG TQLPPQGRTT AHKDPNAIFL SFQHLLRGKV RFLMLVGGST LCVRRAPPTT AVPSRTSLVL TLNEL

Alternative Names

Sonic hedgehog, HHG-1, HHG1, HLP3, HPE3
Product Details
Length 175 aa
Molecular Weight 18.8 kDa
Structure Monomer
Source E. coli
Accession Number P40225
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions. Upon reconstitution, a small amount of visible precipitate can be expected. A 10% overfill has been added to the total material vialed to compensate for this loss.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.