Recombinant Mouse TGF-β3 (Animal-Free)

Code Description Price Qty
GFM27AF-2 Mouse TGF-β3 (Animal-Free), 2 μg $70.00
GFM27AF-10 Mouse TGF-β3 (Animal-Free), 10 μg $180.00
GFM27AF-100 Mouse TGF-β3 (Animal-Free), 100 μg $705.00
GFM27AF-1000 Mouse TGF-β3 (Animal-Free), 1 mg $5,765.00
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human TGF-β3 has a predicted Mw of 25.7 kDa (each monomer is 12.9 kDa).

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PODS® Growth Factors

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Animal-Free

This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.

Description

Transforming Growth Factors (TGFs) are multifunctional peptides that regulate growth and differentiation in most cell types. The TGF-β family of proteins signal through serine/threonine kinase receptors. TGF-β isoforms (TGF-β1, -β 2, and -β 3) have overlapping, yet distinct biological actions in developing and adult tissues. TGF-β3 is an important factor in regulating cell adhesion and accelerating wound repair. TGF-β3 also functions during osteoblast proliferation, chemotaxis, and collagen synthesis.

Activity Assay

Mouse TGF-β3 (Animal-Free) is fully biologically active when compared to standard. The activity is determined by the inhibition of IL-4 induced HT-2 cell proliferation and it is typically less than 1 ng/ml. This corresponds to an expected specific activity of 1.0 x 106 units/mg.

AA Sequence

MALDTNYCFR NLEENCCVRP LYIDFRQDLG WKWVHEPKGY YANFCSGPCP YLRSADTTHS TVLGLYNTLN PEASASPCCV PQDLEPLTIL YYVGRTPKVE QLSNMVVKSC KCS

Alternative Names

Transforming Growth Factor β 3, TGF β 3, TGF-β-3, TGFB3, TGFβ3
Product Details
Length 113 / 226 aa
Molecular Weight 12.9 / 25.7 kDa
Structure Dimer
Source E. coli
Accession Number P10600
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation In solution on 10 mM acetic acid and 20% ethanol at a concentration of 0.25 mg/ml
Reconstitution Not applicable
Stability and Storage

12 months from date of receipt when stored at 4°C as supplied.