Recombinant Mouse TNF-α (Animal-Free)
Cytolysis of mouse L929 cells in the presence of Actinomycin D for Mouse TNF-α. Cell viability was measured to calculate the ED50, which is as expected less than 100 pg/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Mouse TNF-α has a predicted Mw of 17.4 kDa.
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Animal-FreeThis product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.
DescriptionTumor Necrosis Factor α (TNF-α) is an inflammatory cytokine secreted by macrophages, monocytes, neutrophils, T cells, and Natural Killer (NK) cells following stimulation by bacterial lipopolysaccharide (LPS). TNF-α signal activation occurs through two receptors, TNFR1 and TNFR2. TNFR1 is expressed on most cell types, unlike TNFR2, which is expressed mainly on immune cells. TNF-α functions to stimulate phagocytosis in macrophages, chemoattract neutrophils, increase insulin resistance, and induce fever.
Activity AssayMouse TNF-α (Animal-Free) is fully biologically active when compared to standard. The activity is determined by the cytolysis of mouse L929 cells in the presence of Actinomycin D and it is typically less than 100 pg/ml. This corresponds to an expected specific activity of 1 x 107 units/mg.
AA SequenceMVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL
Alternative NamesTumor Necrosis Factor α, TNFa, TNFα, TNFSF2, cachectin, DIF, necrosin, cytotoxin, cachexin, TNF
|Molecular Weight||17.5 kDa|
|Purity||≥95% determined by reducing and non-reducing SDS-PAGE|
|Endotoxin Level||≤1.00 EU/μg as measured by kinetic LAL|
|Formulation||Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5|
|Reconstitution||Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.|
|Stability and Storage||
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed.
Avoid freeze-thaw cycles