Recombinant Mouse TPO

Code Description Price Qty
GFM32-10 Mouse TPO, 10 μg $189.00
GFM32-100 Mouse TPO, 100 μg $608.00
GFM32-500 Mouse TPO, 500 μg $2,895.00
GFM32-1000 Mouse TPO, 1 mg $5,010.00
Induced proliferation of MO7e cells assay for Mouse TPO. Cell proliferation was measured to calculate the ED50, which is as expected less than 5 ng/ml.

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Description

Thrombopoietin (TPO) is a growth factor that is produced by liver and kidney tissues. TPO binds the TPO receptor (CD110) to promote megakaryocyte maturation, differentiation, and the production of platelets.

Activity Assay

Mouse TPO is fully biologically active when compared to standard. The activity is determined by the ability to induce MO7e cells proliferation and it is typically less than 5 ng/ml. This corresponds to an expected specific activity of 2.0 x 105 units/mg.

AA Sequence

MSPVAPACDP RLLNKLLRDS HLLHSRLSQC PDVDPLSIPV LLPAVDFSLG EWKTQTEQSK AQDILGAVSL LLEGVMAARG QLEPSCLSSL LGQLSGQVRL LLGALQGLLG TQLPLQGRTT AHKDPNALFL SLQQLLRGKV RFLLLVEGPT LCVRRTLPTT AVPSSTSQLL TLNKF

Alternative Names

TPO, Megakaryocyte Colony Stimulating Factor, c-MPL ligand, MGDF
Product Details
Length 175 aa
Molecular Weight 18.8 kDa
Structure Monomer
Source E. coli
Accession Number P40226
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions. Upon reconstitution, a small amount of visible precipitate can be expected. A 10% overfill has been added to the total material vialed to compensate for this loss.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.