Recombinant Mouse VEGF-120

Code Description Price Qty
GFM52-2 Mouse VEGF-120, 2 μg $65.00
GFM52-10 Mouse VEGF-120, 10 μg $165.00
GFM52-100 Mouse VEGF-120, 100 μg $475.00
GFM52-1000 Mouse VEGF-120, 1 mg $3,930.00
Induced proliferation of HUVEC cells for Mouse VEGF-120. Cell proliferation was measured to calculate the ED50.

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Description

Vascular Endothelial Growth Factor 120 (VEGF-120) is produced by a wide variety of cell types, including tumor and vascular cells. VEGF-120 is a mediator of vascular growth, vascular permeability, and plays a role in stimulating vasodilation via nitric oxide-dependent pathways. Mouse VEGF-A has several alternatively spliced isoforms, with one being VEGF-120. VEGF-120 is an angiogenic factor that is expressed throughout endochondral bone development and is important during skeletogenesis.

Activity Assay

Mouse VEGF-120 is fully biologically active when compared to standard. The activity is determined by the proliferation of HUVEC cells and it is typically less than 5 ng/ml. This corresponds to an expected specific activity of 2.0 x 105 units/mg.

AA Sequence

MPMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENCDKPR R

Alternative Names

Vascular Endothelial Growth Factor, VEGF121, VEGF
Product Details
Length 121 / 242 aa
Molecular Weight 14.1 / 28.3 kDa
Structure Dimer
Source E. coli
Accession Number P15692-9
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.