Recombinant Mouse VEGF-120
Induced proliferation of HUVEC cells for Mouse VEGF-120. Cell proliferation was measured to calculate the ED50.
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DescriptionVascular Endothelial Growth Factor 120 (VEGF-120) is produced by a wide variety of cell types, including tumor and vascular cells. VEGF-120 is a mediator of vascular growth, vascular permeability, and plays a role in stimulating vasodilation via nitric oxide-dependent pathways. Mouse VEGF-A has several alternatively spliced isoforms, with one being VEGF-120. VEGF-120 is an angiogenic factor that is expressed throughout endochondral bone development and is important during skeletogenesis.
Activity AssayMouse VEGF-120 is fully biologically active when compared to standard. The activity is determined by the proliferation of HUVEC cells and it is typically less than 5 ng/ml. This corresponds to an expected specific activity of 2.0 x 105 units/mg.
AA SequenceMPMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENCDKPR R
Alternative NamesVascular Endothelial Growth Factor, VEGF121, VEGF
|Length||121 / 242 aa|
|Molecular Weight||14.1 / 28.3 kDa|
|Purity||≥95% determined by reducing and non-reducing SDS-PAGE|
|Endotoxin Level||≤1.00 EU/μg as measured by kinetic LAL|
|Formulation||Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)|
|Reconstitution||Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.|
|Stability and Storage||
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.