Recombinant Porcine GM-CSF (Animal-Free)
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Description
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a hematopoietic growth factor produced by endothelial cells, monocytes, fibroblasts, and T cells. GM-CSF stimulates the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells. GM-CSF promotes immune system development and regulates neutrophil function during infection.
Activity Assay
Porcine GM-CSF is fully biologically active when compared to standard. The activity is determined by the dose dependent proliferation of TF-1 cells and it is typically less than 10 ng/ml.
AA Sequence
MAPTRPPSPV TRPWQHVDAI KEALSLLNNS NDTAAVMNET VDVVCEMFDP QEPTCVQTRL NLYKQGLRGS LTRLKSPLTL LAKHYEQHCP LTEETSCETQ SITFKSFKDS LNKFLFTIPF DCWGPVKK
Alternative Names
CSF-2, Pluripoietin-α, MGI-1GM
| Product Details | |
|---|---|
| Length |
128 aa |
| Molecular Weight |
14.5 kDa |
| Structure |
Monomer |
| Source |
E. coli |
| Accession Number |
Q29118 |
| Purity |
≥95% determined by reducing and non-reducing SDS-PAGE |
| Endotoxin Level |
≤1.00 EU/µg as measured by kinetic LAL |
| Formulation |
Lyophilized from a 0.2 µm filtered solution containing 10 mM sodium phosphate, pH 7.5 |
| Reconstitution |
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions. If a precipitate is observed, centrifuge the solution thoroughly and use only the soluble fraction (removing it from the precipitate). A 10% overfill has been added to compensate for any loss of protein in the precipitate. |
| Stability and Storage |
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed. |
