Recombinant Rat Activin A (Animal-Free)

Code Description Price Qty
GFR6AF-10 Rat Activin A (Animal-Free), 10 μg $206.00
GFR6AF-100 Rat Activin A (Animal-Free), 100 μg $671.00
GFR6AF-500 Rat Activin A (Animal-Free), 500 μg $2,895.00
GFR6AF-1000 Rat Activin A (Animal-Free), 1 mg $4,821.00
Cytotoxicity assay for Rat Activin A. Cell viability was measured to calculate the ED50, which is less than 10 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human Activin A has a predicted Mw of 26.2 kDa (each monomer is 13.1 kDa).

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PODS® Growth Factors

This growth factor is also available as PODS® for sustained release, see here.

Animal-Free

This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.

Description

Activin A is a member of the Transforming Growth Factor β (TGF-β) family of proteins with a wide range of biological activities. Activins are produced in many tissue types including the skin, gonads, lungs, and pituitary gland. Activins interact with receptor type I and type II serine/threonine protein kinases, to activate SMAD signaling and regulate diverse cellular functions, such as cell proliferation, differentiation, wound healing, apoptosis, and metabolism. Activin A is a homodimer comprised of two activin β A chains. Rat Activin A shares 100% amino acid sequence identity with human, mouse, porcine, bovine, and feline Activin A proteins.

Activity Assay

Rat Activin A is fully biologically active when compared to standard. The activity is determined by using a cytotoxicity assay using MPC-11 cells and it is typically less than 10 ng/ml. This corresponds to an expected specific activity of 1 x 105 units/mg.

AA Sequence

MGLECDGKVN ICCKKQFFVS FKDIGWNDWI IAPSGYHANY CEGECPSHIA GTSGSSLSFH STVINHYRMR GHSPFANLKS CCVPTKLRPM SMLYYDDGQN IIKKDIQNMI VEECGCS

Alternative Names

Inhibin β-1, FRP, FSH-releasing protein, EDF, erythroid differentiation factor, FRP, follicle stimulating hormone releasing protein, Activin-A
Product Details
Length 117 / 234 aa
Molecular Weight 13.1 / 26.2 kDa
Structure Dimer
Source E. coli
Accession Number P18331
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.