Recombinant Rat EGF

Code Description Price Qty
GFR5-20 Recombinant Rat EGF, 20 μg £50.00
GFR5-100 Recombinant Rat EGF, 100 μg £120.00
GFR5-250 Recombinant Rat EGF, 250 μg £242.50
GFR5-100x10 Recombinant Rat EGF, 100 μg x 10 £465.00
GFR5-1000 Recombinant Rat EGF, 1 mg £465.00
Induced proliferation of BALB/c 3T3 cells assay for Rat EGF. Cell proliferation was measured to calculate the ED50, which is as expected less than 1 ng/ml.

Achieve Purchasing Synergy

Request a quote at quotes@cellgs.com for 2 or more vials of any Growth Factor to receive a discount. Discounts range from 5 to 20% according to the size of your order (offer excludes the largest pack size - please contact us).

PODS Growth Factors

This growth factor is also available as PODS™ for sustained release, see here.

Description

Epidermal Growth Factor (EGF) is a growth factor that stimulates the proliferation, differentiation, and survival of epithelial and epidermal cells. EGF contains three intramolecular disulfide bonds and binds in high affinity to the epidermal growth factor receptor (EGFR). EGF is overexpressed in multiple tumor cell lines and promotes resistance to chemotherapy and radiation treatments.

Activity Assay

Rat EGF is fully biologically active when compared to standard. The activity is determined by the dose-dependent proliferation of BALB/c 3T3 cells proliferation and it is typically less than 1 ng/ml. This corresponds to an expected specific activity of 1.0 x 106 units/mg.

AA Sequence

MNSNTGCPPS YDGYCLNGGV CMYVESVDRY VCNCVIGYIG ERCQHRDLRW WKLR

Alternative Names

Epidermal Growth Factor, pro-epidermal growth factor, urogastrone, URG, β-urogastrone, HOMG4
Product Details
Length 54 aa
Molecular Weight 6.3 kDa
Structure Monomer
Source E. coli
Accession Number P07522
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.