Recombinant Rat IFN-γ

Code Description Price Qty
GFR4-20 Rat IFN-γ, 20 μg $65.00
GFR4-100 Rat IFN-γ, 100 μg $165.00
GFR4-250 Rat IFN-γ, 250 μg $375.00
GFR4-1000 Rat IFN-γ, 1 mg $1,090.00

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PODS® Growth Factors

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Description

Interferon γ (IFN-γ) is a type II interferon that is critical during adaptive and innate immune responses to infection. IFN-γ is produced by T cells and Natural Killer cells following antigen-specific activation. IFN-γ binds IFN-γ receptors (IFN-γ R1 and IFN-γ R2), which are expressed on most immune cells, to activate the JAK-STAT pathway. IFN-γ induced signaling increases the expression of class 1 major histocompatibility complex (MHC) molecules.

Activity Assay

Activity to be determined.

AA Sequence

MQGTLIESLE SLKNYFNSSS MDAMEGKSLL LDIWRNWQKD GNTKILESQI ISFYLRLFEV LKDNQAISNN ISVIESHLIT NFFSNSKAKK DAFMSIAKFE VNNPQIQHKA VNELIRVIHQ LSPESSLRKR KRSRC

Alternative Names

Interferon-γ, type II interferon, T cell interferon, immune Interferon, MAF, IFG, IFI
Product Details
Length 135 aa
Molecular Weight 15.6 kDa
Structure Monomer
Source E. coli
Accession Number P01581
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 100 mM sodium chloride, pH 7.5
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions. If a precipitate is observed, centrifuge the solution thoroughly and use only the soluble fraction (removing it from the precipitate). A 10% overfill has been added to compensate for any loss of protein in the precipitate.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.