Recombinant Rat IL-17E / IL-25
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DescriptionInterleukin-17E (IL-17E), also known as IL-25, is a member of the IL-17 family of cytokines. IL-17E binds to the IL-17BR receptor to stimulate the secretion of the proinflammatory Interleukin-8 (IL-8) chemokine and to induce the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappaB).
Activity AssayRat IL-17E is fully biologically active when compared to standard. The activity is determined by the ability to produce human IL-8 by human PBMC cells.
AA SequenceMCSHLPRCCP SKQQEFPEEW LKWNPAPVSP PEPLRHTHHP ESCRASKDGP LNSRAISPWS YELDRDLNRV PQDLYHARCL CPHCVSLQTG SHMDPMGNSV PLYHNQTVFY RRPCHGEQGA HGRYCLERRL YRVSLACVCV RPRMMA
Alternative NamesInterleukin-17E, interleukin 17E, IL17E, IL 17E, Interleukin-25, interleukin 25, IL25, IL 25, IL-25
|Length||146 / 292 aa|
|Molecular Weight||16.9 / 33.8 kDa|
|Purity||≥95% determined by reducing and non-reducing SDS-PAGE|
|Endotoxin Level||≤1.00 EU/μg as measured by kinetic LAL|
|Formulation||Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)|
|Reconstitution||Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile 10 mM HCI at 0.1 mg/ml, which can be further diluted into other aqueous solutions.|
|Stability and Storage||
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.