Recombinant Rat MCSF

Code Description Price Qty
GFR33-2 Rat MCSF, 2 μg $65.00
GFR33-10 Rat MCSF, 10 μg $165.00
GFR33-100 Rat MCSF, 100 μg $635.00
GFR33-1000 Rat MCSF, 1 mg $5,240.00
Induced proliferation of NFS-60 cells assay for Rat MCSF. Cell proliferation was measured to calculate the ED50, which is as expected less than 10 ng/ml.

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Description

Macrophage Colony Stimulating Factor (MCSF) is a hematopoietic growth factor that is widely produced by a variety of cells. MCSF stimulates the proliferation and differentiation of hematopoietic stem cells into monocyte and macrophage cell types. MCSF also acts through the colony stimulating factor 1 receptor (CSF1R) to modulate processes involved in immunology, bone metabolism, fertility, and pregnancy.

Activity Assay

Rat MCSF is fully biologically active when compared to standard. The activity is determined by the ability to induce NFS-60 cells proliferation and it is typically less than 10 ng/ml. This corresponds to an expected specific activity of 1.0 x 105 units/mg.

AA Sequence

MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP

Alternative Names

Macrophage Colony Stimulating Factor, M-CSF, MGI-IM, CSF-1
Product Details
Length 156 / 312 aa
Molecular Weight 18.2 / 36.4 kDa
Structure Monomer
Source E. coli
Accession Number P07141
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.