Recombinant Rat MIP-1 α / CCL3

Code Description Price Qty
GFR23-5 Rat MIP-1 α, 5 μg $65.00
GFR23-20 Rat MIP-1 α, 20 μg $165.00
GFR23-100 Rat MIP-1 α, 100 μg $410.00
GFR23-1000 Rat MIP-1 α, 1 mg $2,950.00
Induced chemotaxis of THP-1 cells assay for Rat MIP-1 α. Cells that migrated were counted using a luminescent substrate. Migration over basal levels was reported in response to Rat MIP-1 α starting at 10 ng/ml.

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Description

Macrophage Inflammatory Protein 1 α (MIP-1 α), also known as CCL3, is a cytokine produced by macrophages. MIP-1 α binds the chemokine receptors CCR1, CCR4 and CCR5 to induce inflammatory responses, including the recruitment of granulocytes and neutrophil superoxide production. The MIP-1 α and MIP-1 β heterodimer exhibits antiviral activity against the human immunodeficiency virus 1 (HIV-1).

Activity Assay

Rat MIP-1 α is fully biologically active when compared to standard. The activity is determined by the ability to induce chemotaxis of THP-1 cells.

AA Sequence

ASLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPGV IFLTKRSRQV CADPSEEWVQ KYVSDLELSA

Alternative Names

Monocyte Chemotactic Protein 1, MIP-1 alpha, CCL3
Product Details
Length 70 aa
Molecular Weight 7.8 kDa
Structure Monomer
Source E. coli
Accession Number P10147
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.