PODS™ Human IGF-1
PODS™ TechnologyPODS™ proteins are made using an insect cell expression system in which the active protein is co-expressed alongside polyhedrin carrier protein. Polyhedrin forms microcrystals within insect cells which specifically capture the active protein to form a co-crystal complex. The active protein is captured in its nascent, natively folded form with limited scope for proteolytic degradation. Consequently, excellent levels of bioactivity are observed. The PODS™ co-crystals provide a sustained release mechanism and can be used to functionalize surfaces. For further details, please refer to the PODS™ Technology page.
Product DescriptionThe product contains the polyhedrin protein co-crystalized with Human IGF-1. Insulin-like Growth Factor 1 (IGF-1) is a growth factor that is produced by the liver. IGF-1 production is stimulated by Growth Hormone. IGF-1 binds the insulin-like growth factor 1 receptor (IGF1R) and the insulin receptor to stimulate systemic body growth. IGF-1 is one of the most potent activators of the AKT signaling pathway, which stimulates cell proliferation and inhibits programmed cell death.
PODS™ co-crystals provide a depot of proteins which are steadily secreted. It has been estimated that the biological activity of 50 million PODS™ co-crystals generates the same peak dose as 3.3 μg of standard recombinant protein. However, at 5 days following the start of seeding the PODS™ co-crystals, there are more than 50% of these peak levels still present in the culture system. Ultimately, the amount of PODS™ co-crystals that is optimal for a particular experiment should be determined empirically. Based on previous data, we suggest using 50 million PODS™ co-crystals in place of 3.3 μg of standard growth factor as a starting point."
To control for cross-reactivity with cells or as a negative control, we recommend using PODS™ growth factors alongside PODS™ Empty crystals, as the latter do not contain or release cargo protein.
AA SequenceMADVAGTSNR DFRGREQRLF NSEQYNYNNS KNSRPSTSLY KKAGSPETLC GAELVDALQF VCGDRGFYFN KPTGYGSSSR RAPQTGIVDE CCFRSCDLRR LEMYCAPLKP AKSA
Alternative NamesInsulin-like Growth Factor 1, somatamedin C, mechano growth factor, IGF-IA, IGF-IB, IGF-I, IGFI, insulin-like growth factor I, IGF1A1, insulin-like growth factor IA, insulin-like growth factor IB, MGF2, IBP1
|Molecular Weight||13 kDa|
|Source||Spodoptera frugiperda (Sf9) cell culture|
|Endotoxin Level||<0.06 EU/ml as measured by gel clot LAL assay|
|Formulation||PODS™ were lyophilized from a volatile solution|
PODS™ co-crystals may be reconstituted at 200 million co-crystals/ml in water. 20% glucose has a buoyant density closer to PODS™ co-crystals and can be useful for aliquoting.
PODS™ co-crystals are highly stable when stored in aqueous solution (pH range 6 - 8).
|Stability and Storage||Upon receipt, store at 4°C. PODS™ co-crystals are stable for at least 1 year when dry and 6 months when resuspended.|