PODS® Human IL-3
PODS® proteins are made using an insect cell expression system in which the active protein is co-expressed alongside polyhedrin carrier protein. Polyhedrin forms microcrystals within insect cells which specifically capture the active protein to form a co-crystal complex. The active protein is captured in its nascent, natively folded form with limited scope for proteolytic degradation. Consequently, excellent levels of bioactivity are observed. The PODS® co-crystals provide a sustained release mechanism and can be used to functionalize surfaces. For further details, please refer to the PODS® Technology page.
The product contains the polyhedrin protein co-crystallized with Human IL-3. IL-3 stimulates the proliferation and differentiation of pluripotent hematopoietic stem cells and various lineage-committed progenitors. In addition, IL-3 plays important roles in neuronal cell proliferation, stimulation of mature basophils and the activation of monocytes. Its secretion is predominantly mediated by T lymphocytes and mast cells, signal transduction is mediated by the IL-3 receptor. Human IL-3 does not show bioactivity on murine cells due to significant amino acid divergence between human and murine IL-3.
PODS® co-crystals provide a depot of proteins which are steadily secreted. It has been estimated that the biological activity of 50 million PODS® co-crystals generates the same peak dose as 3.3 µg of standard recombinant protein. However, at 5 days following the start of seeding the PODS® co-crystals, there are more than 50% of these peak levels still present in the culture system. Ultimately, the amount of PODS® co-crystals that is optimal for a particular experiment should be determined empirically. Based on previous data, we suggest using 50 million PODS® co-crystals in place of 3.3 µg of standard growth factor as a starting point.
To control for cross-reactivity with cells or as a negative control, we recommend using PODS® growth factors alongside PODS® Empty crystals, as the latter do not contain or release cargo protein.
This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.
MADVAGTSNR DFRGREQRLF NSEQYNYNNS KNSRPSTSLY KKAGFAPMTQ TTPLKTSWVN CSNMIDEIIT HLKQPPLPLL DFNNLNGEDQ DILMENNLRR PNLEAFNRAV KSLQNASAIE SILKNLLPCL PLATAAPTRH PIHIKDGDWN EFRRKLTFYL KTLENAQAQQ TTLSLAIF
Interleukin-3, IL3, IL 3, Hematopoietic growth factor, Mast cell growth factor, MCGF, Multipotential colony-stimulating factor, Multi-CSF, HCGF, P-cell-stimulating factor
Research Use Only
This product is for Research Use Only.
Spodoptera frugiperda (Sf9) cell culture
<0.06 EU/ml as measured by gel clot LAL assay
PODS® were lyophilized from a volatile solution
PODS® co-crystals may be reconstituted at 200 million co-crystals/ml in sterile PBS. 20% glucose has a buoyant density closer to PODS® co-crystals and can be useful for aliquoting.
PODS® co-crystals are highly stable when stored in aqueous solution (pH range 6 - 8).
|Stability and Storage||
Upon receipt, store at 4°C. PODS® co-crystals are stable for at least 1 year when dry and 6 months when resuspended.