Cell culture

Beyond GFP: a growing and versatile molecular toolbox

Beyond GFP: a growing and versatile molecular toolbox

GFP has given rise to a powerful and versatile molecular toolbox. Cycles of rational design and directed mutagenesis, as well as the isolation of entirely new fluorophores from different species, are continuously pushing the capabilities of fluorescent protein (FP) biosensors to photophysical and biochemical extremes.

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Alternatives to CRISPR-Cas9

Alternatives to CRISPR-Cas9

The impact of CRISPR-Cas9 technology is undeniable. Yet, it is not without limitations. As such, researchers have since adopted modifications to the original technology as well as alternatives that address some of these limitations.

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Free radicals vs. reactive oxygen species: what's the difference?

Free radicals vs. reactive oxygen species: what's the difference?

Free radicals, reactive oxygen species, oxidative stress, oxidation, antioxidants; these terms are used in both scientific and non-scientific contexts, though their meaning and relationships with one another often get confused. These molecules have very important biological roles. First, let’s unravel these terms.

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Uses of microcarriers for expansion of cell culture

Uses of microcarriers for expansion of cell culture

Microcarrier-based biomanufacturing has become well established and now represents a large market across all scales of production. Bioreactors on offer which can use microcarriers range from lab sized, with a few liters of capacity, to factory sized, at hundreds or thousands of liters.

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Organoids II: rise of the assembloids

Organoids II: rise of the assembloids

A new wave of innovation is leading to the development of a 2nd generation of ever-more complex organoids, known as assembloids, that will enable more powerful studies to be conducted within the confines of a culture dish allowing greater insights and reducing our reliance on animal models.

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