How sample type, volume and membrane choice set the real capacity of a tangential flow filtration run, from conditioned media and biofluids to viral vectors, nucleic acids, proteins and synthetic nanoparticles, at laboratory and pilot scale
Most tangential flow filtration (TFF) protocols for extracellular vesicles (EVs) use the membrane as a net. Everything bigger than the cut-off stays in the retentate, everything smaller goes to waste, and the EVs come out as a single concentrated population. TFF can also be used to gate EVs based on their size.
EV storage is a topic we are often asked about: Which buffers, what temperatures, and for how long? What is the impact on downstream applications of the EVs?
Genetic audits are regularly carried out to determine the integrity of cells used in research. The utility of these audits depends on the question being asked.
Flux and pressure are process-critical parameters that change during tangential flow filtration (TFF). This article defines mechanisms, explains what rising pressure during a run actually tells you, and addresses the practical question of hollow fibre cartridge orientation.
We compare the criteria that actually determine delivery outcomes: cargo loading, targeting, immune handling, manufacturability, analytical control and regulatory precedent.
Why is SEC becoming more popular, and when is ultracentrifugation still useful?
A question that surfaces repeatedly in extracellular vesicle (EV) method development is whether two desirable properties can be held at the same time: a complete, unbiased EV population isolated without affinity capture, and a preparation that carries no risk of contaminating virus.
Diafiltration, the process of adding fresh buffer to a sample while simultaneously removing permeate, extends the tangential flow filtration technique into a far more powerful tool
The combination of Tangential Flow Filtration (TFF) and size exclusion chromatography (SEC) is a widely used workflow for the isolation of virus-like proteins (VLPs).
