Exosomes

The isolation of extracellular vesicles (EVs) and exosomes is one of the most critical and variable steps in any EV research workflow. The most commonly used non-affinity isolation methods are ultracentrifugation (UC), size exclusion chromatography (SEC), and tangential flow filtration (TFF). The method you choose directly determines the purity, yield and integrity of your isolated vesicles, and has downstream consequences for every subsequent characterisation step, including nanoparticle tracking analysis (NTA), western blot and functional assays

By analysing tumour-derived material circulating in blood, urine, or other body fluids, liquid biopsies offer a minimally invasive window into cancer biology in real time. Among the various analytes being explored, including circulating tumour cells (CTCs) and cell-free DNA (cfDNA), extracellular vesicles (EVs) are emerging as particularly compelling biomarker candidates.

A landmark study defines the protein and lipid signatures that distinguish authentic small extracellular vesicles in human plasma.

How TFF works, filter formats compared, and why EVlution belongs on your bench

EVlution, a new affordable bench top Tangential Flow Filtration (TFF) system launches today. It makes scalable, reproducible extracellular vesicle purification accessible to more laboratories, accelerating innovation in EV research and translational development.

Embryo implantation is a tightly timed conversation between embryo and endometrium; one that has to coordinate adhesion, immune tolerance, tissue remodelling, and blood-vessel changes within a narrow window of implantation. A major set of words in that conversation are extracellular vesicles (EVs), including exosomes; nano-sized packages released by cells that carry proteins, lipids, and nucleic acids (like miRNAs) to influence recipient cells.

If cytokines and growth factors send clear signals, extracellular vesicles (EVs) are complex chords. They have emerged as a powerful and versatile mode of communication. But how clear can the instructions from these complex particles be?

Since emerging in the last 20 years, expectations for exosomes have developed into hype in some areas. What is their real potential?

There are a wide variety of technologies available to concentrate and purify EVs and exosomes. Choosing the right one for your sample and downstream application is critical.

EVs are best known in the context of mammalian systems, where exosomes and microvesicles play critical roles in intercellular communication. However, plants also release vesicle-like particles with intriguing parallels, and key differences, from their animal counterparts.