Green fluorescent protein: GFP legend and legacy

Green fluorescent protein (GFP) was isolated from the jellyfish Aequorea victoria in the early 1960s. On its way to earning Osamu Shimomura, Martin Chalfie and Roger Tsien a Nobel prize in 2008, this protein and variants derived from it have illuminated countless scientific explorations and shed light on many previously dark secrets of biology, proving almost indispensable in life science research. A plethora of variants and new fluorescent proteins are building on the legacy of this versatile molecular biology tool.