Cell Guidance Systems Blog

Why is SEC becoming more popular, and when is ultracentrifugation still useful?

Why self-assembling peptide hydrogels flow under shear and re-heal after it: the mechanism, the rheology tests that matter, and rules for injection and printing.

This article sets out the four classes of composite, explains why charge compatibility decides whether a blend succeeds or precipitates, and gives a practical route to designing and characterising a composite based on PeptiGel.

A question that surfaces repeatedly in extracellular vesicle (EV) method development is whether two desirable properties can be held at the same time: a complete, unbiased EV population isolated without affinity capture, and a preparation that carries no risk of contaminating virus.

Diafiltration, the process of adding fresh buffer to a sample while simultaneously removing permeate, extends the tangential flow filtration technique into a far more powerful tool

All biomedical researchers want to see their work make a real-world impact. The odds are stacked against some fields

The combination of Tangential Flow Filtration (TFF) and size exclusion chromatography (SEC) is a widely used workflow for the isolation of virus-like proteins (VLPs).

Matrigel is widely used to support inoculated cancer cells as they establish tumours in mouse cancer models. But the poorly defined nature of Matrigel, and the high levels of cytokines it contains, may be confounding the interpretation of data.

A systematic look at how to optimize TFF for EV purification: what each parameter controls, how to set it, and how to confirm that the resulting preparation meets the quality standards that downstream work, whether cargo analysis, functional assay or therapeutic manufacture, demands.

Three analytical layers dominate EV analysis. Proteomics catalogues the proteins, transcriptomics profiles the RNA, and lipidomics maps the membrane lipids. Together they form the core of EV cargo analysis.