Cell Guidance Systems Blog

Diafiltration, the process of adding fresh buffer to a sample while simultaneously removing permeate, extends the tangential flow filtration technique into a far more powerful tool

All biomedical researchers want to see their work make a real-world impact. The odds are stacked against some fields

The combination of Tangential Flow Filtration (TFF) and size exclusion chromatography (SEC) is a widely used workflow for the isolation of virus-like proteins (VLPs).

Matrigel is widely used to support inoculated cancer cells as they establish tumours in mouse cancer models. But the poorly defined nature of Matrigel, and the high levels of cytokines it contains, may be confounding the interpretation of data.

A systematic look at how to optimize TFF for EV purification: what each parameter controls, how to set it, and how to confirm that the resulting preparation meets the quality standards that downstream work, whether cargo analysis, functional assay or therapeutic manufacture, demands.

Three analytical layers dominate EV analysis. Proteomics catalogues the proteins, transcriptomics profiles the RNA, and lipidomics maps the membrane lipids. Together they form the core of EV cargo analysis.

How neuron-derived extracellular vesicles (NDEVs) carry amyloid-beta, tau and alpha-synuclein between neurons and provide blood biomarkers for Alzheimer's, Parkinson's, ALS and FTD, plus the growth factor and EV isolation tools that support the work.

This article surveys the growth factors that underpin organoid culture, why they matter, and how their delivery shapes organoid quality, reproducibility and cost. It explains the roles of the core signalling families, why stem-cell niches depend on tightly regulated factor gradients, and how the practical challenges of growth factor instability and expense are driving interest in sustained-release approaches.

How polyhedrin functions as a viral survival strategy, and how the PODS (Polyhedrin Delivery System) mechanism harnesses polyhedrin's crystalline architecture to encapsulate growth factors and control their release over days to weeks.