Cell Guidance Systems Blog

A major challenge of working with exosomes and other types of extracellular vesicles (EVs) is their characterization and agreeing parameters that define each group. Recently, this task has become even more challenging with a dawning realization that proteins (and nucleic acids) loosely associated with the surface of exosomes, once thought to be artefacts of purification, are functionally important.

Purified recombinant proteins are used in products ranging from biological soap powders to cutting-edge medicines. The rate at which these proteins degrade is critical to their function. Technologies that address the rate of degradation and enable novel applications can transform the value of a protein

The tumour microenvironment (TME) is a dynamic, highly heterogeneous structure consisting of both transformed (mutated) cells, non-transformed cells (including immune cells, stromal cells and blood vessels) and microbes. These cells are held in an extracellular matrix of proteins and other factors secreted by the cells. An increased understanding of the TME is behind many of the latest advancements in cancer therapy.

The recent emergence of genetic therapies has focussed attention on exosomes as a possible mechanism for their efficient delivery. Exosomes provide an efficient, natural mechanism for transferring RNA into cells. Exosomes are also durable, have low levels of immunogenicity and can be produced economically at scale. The biggest hurdle to the widespread adoption of exosomes as delivery vehicles is their low cargo-loading efficiency.

Cochlear implants (CIs) do not produce the same rich sound experience that can be provided by properly functioning ears. One limitation on performance is the quality of connection CIs form with auditory nerves they electrically stimulate. This connection may be improved by co-implanting iPSC-derived otic neuronal progenitor cells to help bridge the gap. A “neurotrophic strip” containing slowly releasing growth factors that generate a growth factor gradient ensures these neurons extend in the right direction.

Cell Guidance Systems is now offering a range of high-quality and well-characterized freeze-dried exosome samples from human cancer cell lines for research applications. These Instant exosomes are isolated using Exo-spin kits following a combination of precipitation and size exclusion chromatography (SEC).

In order to study lipids, they must often be extracted first from tissues or cellular cultures and then quantified. There are various methods available for lipid quantification, ranging from state-of-the-art quantification using advanced technology to simpler benchtop solutions. But which technique is relevant for you?

Anti-oxidants, omega-3, calcium and vitamins etc, have been linked to health benefits. Producers of foods containing these nutrients highlight these health-giving properties to promote sales. Might exosomes soon be added to the list? A growing understanding of the role of extracellular vesicles (EVs) points to the remarkable potential of food-derived exosomes (FDEs) as a distinct dietary component with nutritional benefits.

Chromosomal alterations underlie numerous medical conditions, with clinical detection of chromosome changes being a key feature of genetic diagnostics. Chromosomal alterations are also important in research, affecting the behaviour of cells and impacting the interpretation of data generated with them.

The goal of precision medicine is to understand the factors that contribute to the variability in the pharmacokinetics and pharmacodynamics of the drugs. It is now clear that the gut microbiome plays a critical role in the markedly different responses of individuals to identical drug therapy.