A question that surfaces repeatedly in extracellular vesicle (EV) method development is whether two desirable properties can be held at the same time: a complete, unbiased EV population isolated without affinity capture, and a preparation that carries no risk of contaminating virus.
Diafiltration, the process of adding fresh buffer to a sample while simultaneously removing permeate, extends the tangential flow filtration technique into a far more powerful tool
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The combination of Tangential Flow Filtration (TFF) and size exclusion chromatography (SEC) is a widely used workflow for the isolation of virus-like proteins (VLPs).
