A recent paper exploring the use of optimized virus like particles (VLPs) to deliver base editing proteins has shown impressive levels of efficacy and, importantly, low levels of off-target activity in mouse models of therapy for brain, eye and liver disease.
In this report, we provide details on 45 companies that have emerged over the last decade to pursue exosome therapeutics and diagnostic goals. Two tables provide a quick reference for this information on investor funding and founding date. A further table provides a summary of 242 exosome-focused clinical trials.
Tissue development and homeostasis relies on the availability of spatiotemporal reference points provided by localized variations in physical and chemical parameters. These create gradients along which cells can move and be maintained. Durotaxis is a less well-known but important mechanism by which cells move along a gradient of elasticity (stiffness).
Therapeutic RNA delivery can be accomplished by a variety of viral or non-viral methods. The type of RNA structure carried by these diverse delivery methods also varies (e.g., oligonucleotides, miRNA, siRNA, lncRNA, mRNA, saRNAs). The particular delivery method used is dependent on the types of RNA and the target and is a hugely important consideration for the development of effective drugs.
GFP has given rise to a powerful and versatile molecular toolbox. Cycles of rational design and directed mutagenesis, as well as the isolation of entirely new fluorophores from different species, are continuously pushing the capabilities of fluorescent protein (FP) biosensors to photophysical and biochemical extremes.
Green fluorescent protein (GFP) was isolated from the jellyfish Aequorea victoria in the early 1960s. On its way to earning Osamu Shimomura, Martin Chalfie and Roger Tsien a Nobel prize in 2008, this protein and variants derived from it have illuminated countless scientific explorations and shed light on many previously dark secrets of biology, proving almost indispensable in life science research. A plethora of variants and new fluorescent proteins are building on the legacy of this versatile molecular biology tool.
Each year, 1.27 million deaths are caused by drug-resistant microbes. These bugs are currently developing resistance at a faster rate than new drugs are developed. In 30 years’ time, if this innovation gap continues, 10 million people are forecast to die each year from infections that were once treatable. Such a toll would surpass even cancer as a cause of mortality. With such a grave threat to human health, why isn’t more being done? Why aren’t more antibiotics being developed?
The immune response is orchestrated. There are mechanisms to activate and deactivate activities. Myeloid-derived suppressor cells (MDSCs) are immune cells that act as regulators of immune responses. They are important in several diseases including tumor growth and the response to cancer therapies, Graft-Versus-Host Disease (GVHD), various autoimmune diseases, and COVID-19. Novel drugs developed to modulate MDSCs are showing promise with ongoing clinical trials for COVID-19 and some cancers.
The impact of CRISPR-Cas9 technology is undeniable. Yet, it is not without limitations. As such, researchers have since adopted modifications to the original technology as well as alternatives that address some of these limitations.
Increasingly, the key roles of biophysical cues in modulating stem cell response have been studied in vitro. Based on the ability of cells to actively sense and react to their microenvironment through mechanotransduction systems, these studies have shown that the growth and differentiation of stem cells can be controlled—even in the absence of biochemical stimuli such as growth factors. These reports further suggest that the stimulation delivered by biophysical cues actually have advantages over biochemical stimuli.
